RNA interference (RNAi) is a post transcriptional silencing process of sequence specific genes that widely exists in organisms caused by double stranded RNA homologous to the target gene sequence.
  Because RNAi has high sequence specificity, it can specifically silence specific genes, so as to obtain the loss of gene function or the reduction of gene expression. Therefore, it can be used as a powerful tool for functional genomics research. RNAi technology can be widely used in functional science, drug target screening, cell signal transduction pathway analysis, disease treatment and so on.
一、RNAi interferes with lentivirus
  1. Design and synthesize interference sequences for target genes;
  2. Select the appropriate carrier;
  3. Construct RNAi interference lentivirus vector and sequence it to verify that it is correct;
  4. Incorrect extraction and sequencing of recombinant plasmids containing endotoxin;
  5. 293T cells were co transfected with high efficiency recombinant vector and lentivirus packaging plasmid, virus packaging was carried out, and the supernatant was collected;
  6. The virus was concentrated and purified by ultrafiltration and ultracentrifugation;
  7. 293T cells were infected with virus solution and the virus titer was measured.
二、Result provision
  1. Provide 1m interfering lentivirus and 1m negative control. The virus titer is 21 * 108tu / MI
  2. Provide lentivirus titer test report
三、Comparison among lentivirus, adenovirus and retrovirus vector systems

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Molecular biology, cell biology, pathological staining detection, cell knockout / knock-in, model and transgenic animals, SPF animal conservation, immunological detection, imaging detection, primary culture, cell drug screening, CRISPR / cas9 gene editing, microarray, high-throughput sequencing, leucomics, metabolomics, high-throughput high-content screening, pharmacodynamic evaluation, pharmacological and toxicological experiments, lentivirus packaging and establishment of stable transgenic strains SiRNA and nano drug loading, chip, CO IP, bioinformatics analysis, intellectual property services!