Mass spectrometry technical services
inputtime:2020-07-17 hit:


China Life Science Experiment Technology Service Alliance bow into the liquid chromatography-mass spectrometer of Agilent company of the United States. A large-scale protein analysis support technology system with high throughput, high sensitivity and high resolution has been formed. In order to promote the development of proteomics and related research and share advanced technical resources, a number of mature analytical technologies in biological mass spectrometry laboratory, protein / peptide mass spectrometry identification, bioengineering drug structure confirmation and proteomics have provided extensive services for customers at home and abroad in recent years.
TSQ mass spectrometer of American Thermal Power Company; The mass spectrometer uses ESI (electrospray ionization) ion source and MS/MS tandem quadrupole analyser. It can detect the actual molecular weight of polar organic compounds, nucleic acids, polypeptides, small molecular proteins and their chemically modified products online, and conduct molecular identification, purity and modification effect analysis on the submitted samples.
Quality range M / Z 10 ~ 2000 resolution r = 2m

Test scope:Molecular weight determination of peptides and proteins by electrospray ionization mass spectrometry and mass spectrometry of natural products, macromolecules and organic molecules.
Requirements for submitted samples:The sample shall be free of salt, ionic surfactant, descaling agent and - some non volatile salts as far as possible. The sample delivery quantity, dissolution method and samples whose mass spectrometry detection is unknown can be contacted with our staff in order to improve the detection efficiency. (please attach the product name of the test sample and accurately calculate the molecular weight)
Instructions for sample delivery:
A: Sample quantity: 50pmol dry powder or above 1ug / UL (general test), 100pmo dry powder or above 1ug / UL (MALDI tandem mass spectrometry analysis), 100ug dry powder or above (disulfide bond analysis, glycosylation analysis, * * acidification analysis, other modification analysis), 10ug dry powder or above (SDS-PAGE or IEF);
B: Salt content: volatile inorganic salt, < 20 mm. Do not use mass spectrum interfering substances such as PBS, SDS and urea;
C: All samples dyed with Coomassie brilliant blue can be accepted, and glutaraldehyde shall not be used as fixative in the process of silver dyeing;
D: Please inform whether the sample is modified and indicate the modification method.
Main applications:Proteomics research; Study on oligosaccharides and glycoproteins; Small molecule compound analysis; Drug discovery and research and development; Analysis of food and environmental samples.
The test report includes the following results:Primary mass spectrometry (mass spectrum, peptide molecular weight, database search results); Secondary mass spectrometry (mass spectrum, peptide molecular weight, database search results, peptide amino acid sequence, modification sites, etc.).
[service process]
[biological services]

[platform cooperation]

Molecular biology, cell biology, pathological staining detection, cell knockout / knock-in, model and transgenic animals, SPF animal conservation, immunological detection, imaging detection, primary culture, cell drug screening, CRISPR / cas9 gene editing, microarray, high-throughput sequencing, leucomics, metabolomics, high-throughput high-content screening, pharmacodynamic evaluation, pharmacological and toxicological experiments, lentivirus packaging and establishment of stable transgenic strains SiRNA and nano drug loading, chip, CO IP, bioinformatics analysis, intellectual property services!



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