The synthetic polypeptide of qianzhaoxinye biological center adopts the step-by-step solid-phase synthesis method, and the reaction system is treated by microwave technology, which can synthesize long complex polypeptide. Polypeptide synthesis is a process of repeatedly adding amino acids. One end of the polypeptide is fixed on a special support, and a unified polypeptide is finally formed through circulation steps.
Peptide chain design:
Polypeptide is a complex macromolecule, and the sequence is unique in physical and chemical properties. When designing polypeptide, the difficulties in the process of synthesis, purification and dissolution should be considered, so that the polypeptide can be used to achieve experimental purposes.
How to reduce the difficulty of peptide chain synthesis:1. Reduce sequence length; 2. Reduce hydrophobic residue number; 3. Reduce difficult residues.
How to enhance the solubility of peptide chains:1. Change N-terminal or C-terminal; 2. Shorten or lengthen the sequence; 3. Adding soluble residues; 4. Changing the sequence by replacing one or more residues; 5. Change the sequence by selecting different frames.
Polypeptide preservation:
1. Lyophilized polypeptides can be stored at 20 degrees or room temperature;
2. The peptide in solution is far more unstable than that in lyophilized form, and the solution should be kept at neutral pH (5-7) - 20 degrees;
3. In order to avoid repeated freezing and thawing of samples, it is best to store them in small samples.
Use of polypeptide:
It is mainly used for the preparation of antibodies, peptide vaccines and peptide micro injections to study the relationship between gene structure and function. The center provides polypeptides with different purity, and most of the crude products are up to 98% of the pharmaceutical grade. Customers can choose according to their needs.
>80%: immunological application, polyclonal antibody preparation
>90%: SAR study, bioassay
>95%: NMR, crystallization, in vitro bioassay
>98%: NMR, crystallization, sensitive bioassay
Scope of services:
1) Common linear peptide, chain length up to 189 amino acids, gram to gram level, purity up to 99%
2) Simple modified peptide: free N-terminal Z acylation and terminal amidation
3) Complex modified peptides: lipopeptide, phosphopeptide (SER, THR, Tyr), cyclic peptide (amide ring, disulfide bond ring), fluorescent labeled peptide (FAM, FITC), biotin labeled peptide (biotin), complex antigen peptide (map), etc;
4) Protein crosslinking: KLH, BSA, ova
5) Peptides containing special amino acids: containing D-type amino acids and various amino acid derivatives;
6) Different purity ranges (crude peptide, > 70%, > 80%, > 90%, > 95%, > 98%, > 99%).
[service process]
[biological services]
[platform cooperation]
Molecular biology, cell biology, pathological staining detection, cell knockout / knock-in, model and transgenic animals, SPF animal conservation, immunological detection, imaging detection, primary culture, cell drug screening, CRISPR / cas9 gene editing, microarray, high-throughput sequencing, leucomics, metabolomics, high-throughput high-content screening, pharmacodynamic evaluation, pharmacological and toxicological experiments, lentivirus packaging and establishment of stable transgenic strains SiRNA and nano drug loading, chip, CO IP, bioinformatics analysis, intellectual property services!
