The synthetic polypeptide of qianzhaoxinye biological center adopts the step-by-step solid-phase synthesis method, and the reaction system is treated by microwave technology, which can synthesize long complex polypeptide. Polypeptide synthesis is a process of repeatedly adding amino acids. One end of the polypeptide is fixed on a special support, and a complete polypeptide is finally formed through the circulation step.

The center can also provide other series of modification services, such as phosphorylation, coupling KLH and BSA, amination, acetylation, biotin and FITC labeling.

Polypeptides are complex macromolecules, and each sequence is unique in physical and chemical properties. When designing polypeptides, the difficulties in the process of synthesis, purification and dissolution should be considered, so that the polypeptides can be used to achieve experimental purposes.

How to reduce the difficulty of peptide chain synthesis: 

1. Reduce the sequence length; 

2. Reduce hydrophobic residue number; 

3. Reduce difficult residues.

How to enhance the solubility of peptide chains: 

1. Change the N-terminal or C-terminal; 

2. Shorten or lengthen the sequence; 

3. Adding soluble residues; 

4. Changing the column by replacing one or more residues; 

5. Change the sequence by selecting different frames.

Polypeptide preservation:

1. Freeze dried polypeptides can be stored at 20 ° C or room temperature; 2. The peptide in solution is far more unstable than that in lyophilized form, and the solution should be kept in neutral pH (5-7) - 20C; 3. In order to avoid repeated freezing and thawing of samples, it is best to store them in small samples.

Use of polypeptide:

>98%: NMR, crystallization, sensitive bioassay

Scope of services:

6) Different purity ranges (crude peptide, > 70%, > 80%, > 90%, > 95%, > 98%, > 99%)

[service process]

Molecular biology, cell biology, pathological staining detection, cell knockout / knock-in, model and transgenic animals, SPF animal conservation, immunological detection, imaging detection, primary culture, cell drug screening, CRISPR / cas9 gene editing, microarray, high-throughput sequencing, leucomics, metabolomics, high-throughput high-content screening, pharmacodynamic evaluation, pharmacological and toxicological experiments, lentivirus packaging and establishment of stable transgenic strains SiRNA and nano drug loading, chip, CO IP, bioinformatics analysis, intellectual property services!