Unlabeled mass spectrometry profiling (L Abel free) only needs to analyze the mass spectrometry data generated during large-scale identification of proteins, and quantify them with the mass spectrometry data of corresponding peptide segments of proteins.
Non labeled quantitative technology has been highly praised by many scientists in quantitative proteomics research. The principle is to analyze the quantitative changes of proteins in samples from different sources by comparing the times of mass spectrometry analysis or mass spectrometry peak intensity. It believes that the frequency of peptide capture and detection in mass spectrometry is positively correlated with its abundance in the mixture. Therefore, the counting of proteins detected by mass spectrometry reflects the abundance of proteins. Through appropriate mathematical formula, the counting of mass spectrometry detection can be linked with the amount of proteins, so as to quantify proteins.
Technical features:
There is no need for expensive stable isotope labeling samples, and the experimental cost is low;
Few operations on samples;
Not subject to sample conditions
It requires high stability and repeatability of liquid chromatography tandem mass spectrometry;
Correct spectrum correction is required, otherwise its quantitative accuracy and reproducibility willbe affected
The dynamic range of detectable peptides can reach more than 4 orders of magnitude, which improves the detection efficiency and accuracy of low abundance proteins.
Application:
Screening of disease markers;
Drug action target research;
Screening of special functional proteins;
[service process]
[biological services]
[platform cooperation]
Molecular biology, cell biology, pathological staining detection, cell knockout / knock-in, model and transgenic animals, SPF animal conservation, immunological detection, imaging detection, primary culture, cell drug screening, CRISPR / cas9 gene editing, microarray, high-throughput sequencing, leucomics, metabolomics, high-throughput high-content screening, pharmacodynamic evaluation, pharmacological and toxicological experiments, lentivirus packaging and establishment of stable transgenic strains SiRNA and nano drug loading, chip, CO IP, bioinformatics analysis, intellectual property services!
