Associated vector of molecular cloning
The cloning of DNA fragments requires appropriate vectors, vectors or plasmids, phages or viruses, which are usually artificially modified. As a carrier, it must have two conditions: first, the carrier must be able to replicate independently in the cell, that is, it must have the origin of replication; Second, the vector must have suitable enzyme digestion sites, and these enzyme digestion sites are not in the replication origin region. The above two ensure the reproduction and availability of the carrier. In order to obtain positive clones, there are often screening markers on the vector, such as resistance to antibiotics, color reaction of some gene products and so on.
Service requirements
1. Please inform us of the main purpose and specific requirements of the service by email.
2. Provide the source and background information of exogenous DNA. DNA sources include: PCR amplification products; RT - PCR amplification products; DNA fragments obtained from other plasmids.
3. Provide electrophoresis photos of DNA fragments and indicate the concentration of marker.
4. Provide carrier source and background information.
5. Indicate the enzyme digestion site used for cloning and whether flat end connection is required.
Description of relevant materials
1. Dangerous materials involving pathogens in experimental materials are not acceptable.
2. Please send the base sequence involved in the experiment to our center in the form of email.
3. The carrier used in the experiment should provide background information as much as possible: (plasmid size, resistance, copy number, polyclonal site, etc.) if it is a commercial vector, please provide the manufacturer and standard name; If the provided carrier is built by yourself, please indicate the general structure.
4. Our center can provide antibiotics other than those commonly used (ampicillin, chlorin and kanamycin), which you need to provide.
5. If the primer you provided is dry, please mark the specific od number. If it is liquid, please mark the concentration.
6. The bacteria can be mailed in the form of glycerol bacteria and puncture bacteria. (it is better to provide corresponding plasmids at the same time); If plasmids are provided, the amount of plasmids should preferably be more than 4ug; If PCR products are provided, the amount of PCR products should preferably be more than 5u (electrophoretic map should be attached).
7. The materials used to extract RNA / DNA should be fresh. When sampling, it should be quickly frozen in liquid nitrogen, stored in a - 80 degree refrigerator and transported with dry ice.
Result provision
1. Relevant experimental instructions and relevant enzyme digestion pictures.
2. Sequencing results.
3. The constructed carrier and bacterial solution are - parts.
4. If there is any identification, the customer shall provide PCR identification pictures.
[service process]
[animal models that can be built on this platform (part)]
[platform cooperation]
Molecular biology, cell biology, pathological staining detection, cell knockout / knock-in, model and transgenic animals, SPF animal conservation, immunological detection, imaging detection, primary culture, cell drug screening, CRISPR / cas9 gene editing, microarray, high-throughput sequencing, leucomics, metabolomics, high-throughput high-content screening, pharmacodynamic evaluation, pharmacological and toxicological experiments, lentivirus packaging and establishment of stable transgenic strains SiRNA and nano drug loading, chip, CO IP, bioinformatics analysis, intellectual property services!
