In the past, laboratories usually used PCR and RT - PCR methods to obtain a gene fragment, but they often couldn't catch the gene because of samples and templates. Now, we use chemical synthesis to artificially construct gene fragments, that is, whole gene synthesis and splicing technology. It can serve the majority of scientific research friends.
Gene synthesis refers to a technology of synthesizing double stranded DNA molecules in vitro, which is different from oligonucleotide synthesis. Oligonucleotides are single stranded, and the longest fragment that can be synthesized is only about 100nt, while gene synthesis is double stranded DNA molecular synthesis, and the length range that can be synthesized is 50bp-12kb. According to the needs of customers, our center can provide whole gene synthesis services with high speed, short cycle and favorable price. Complete gene sequencing reports can be provided to different strains according to the needs of customers.
Gene synthesis refers to a technology of synthesizing double stranded DNA molecules in vitro, which is different from oligonucleotide synthesis. Oligonucleotides are single stranded, and the longest fragment that can be synthesized is only about 100nt, while gene synthesis is double stranded DNA molecular synthesis, and the length range that can be synthesized is 50bp-12kb. According to the needs of customers, our center can provide whole gene synthesis services with high speed, short cycle and favorable price. The synthesized genes can be connected to different vectors according to the needs of customers. The final products include strains, lyophilized plasmids and complete sequencing reports of the whole gene.
Scope of application of whole gene synthesis:
A: Genes in nature that cannot be obtained by conventional experimental means;
B: Researchers design gene sequences through their own will to obtain genes that do not exist in nature;
C: A large number of codon optimization and rearrangement of known gene sequences are carried out in order to achieve the purpose of high-efficiency expression of the gene or obtaining new biological characteristics.
Service advantage
A: The longest synthetic gene has reached 10.5kb, and more than 10000 genes have been completed;
B: Repeats can be synthesized (unlimited number of repeats) with high GC (less than 80%) Hairpin (refer to the limit of less than 3 hairpins per 200bp). Genes rich in special structures such as continuous single base repeats (GC less than 15 and at less than 20);
Note: the data related to the synthesis of special structure genes are summarized according to the gene sequences successfully synthesized by our company, which can be used as a reference to judge whether the gene sequence can be synthesized smoothly when you design the gene sequence. The details need to be determined by the actual gene sequence.
C: The gene can be cloned into the expression vector provided or designated by the customer;
D: Provide free consulting services related to gene synthesis, such as gene structure design, gene codon optimization, gene cloning and expression scheme design.
Results provided:
A: Gene synthesis report -- copies
B: Plasmid containing target gene (not less than 1ug) - copies
C: Strains containing target genes - copies
D: A sequencing map of gene synthesis
[service process]
[animal models that can be built on this platform (part)]
[platform cooperation]
Molecular biology, cell biology, pathological staining detection, cell knockout / knock-in, model and transgenic animals, SPF animal conservation, immunological detection, imaging detection, primary culture, cell drug screening, CRISPR / cas9 gene editing, microarray, high-throughput sequencing, leucomics, metabolomics, high-throughput high-content screening, pharmacodynamic evaluation, pharmacological and toxicological experiments, lentivirus packaging and establishment of stable transgenic strains SiRNA and nano drug loading, chip, CO IP, bioinformatics analysis, intellectual property services!
